Journal: Chemical Biology & Drug Design

Article Title: Investigation on the antipyretic mechanism of Chaiqin Qingning capsule for the treatment of fever based on network pharmacology, molecular docking, and in vitro experimental validation

doi: 10.1111/cbdd.14451

Figure Lengend Snippet: FIGURE 7 Western blot detection of CQQNC on the expression of COX-1, COX-2, cAMP, EP3, and mPGES1. Statistical significance: ∆∆∆∆p < .0001, ∆∆p < .01, and ∆p < .05 represents IL-1β group versus control group. ****p < .0001, **p < .01, and *p < .05 represent CQQNC group versus IL-1β group (n = 3).

Article Snippet: AntiCOX- 1 (batch number: 00100314), COX- 2 (batch number: 10027896), EP3 (batch number: 00071265), mouse and rabbit GAPDH (batch number: 10021642 and 0013796) antibody were purchased from Proteintech.

Techniques: Western Blot, Expressing, Control

Journal: Cell communication and signaling : CCS

Article Title: PTGER4 signaling regulates class IIa HDAC function and SPINK4 mRNA levels in rectal epithelial cells.

doi: 10.1186/s12964-024-01879-1

Figure Lengend Snippet: Fig. 5 PGE2-PTGER4 activates HDACs to increase SPINK4 expression. Western blot analysis for A EP4i, B PGE2 or EP4i, or C butyrate-treated organoid protein lysates probed for HDAC4 (S246)/ HDAC5 (S259)/ HDAC7 (S155), HDAC 4, and GAPDH as a loading control. The experiment in panel A is representative of an experiment that was performed on 2 different patients. Panels B and C are representative of experiments performed on 3 different patient samples. D RT-PCR fold-change quantification of SPINK4 normalized to β-ACTIN in organoids treated with PGE2 and/or LMK-235 (n = 5 different patient samples were used in this experiment). ns = non-significant; *P < 0.05; **P < 0.01; Two-way ANOVA

Article Snippet: For detecting PTGER4 by Western blotting, rabbit anti-PTGER4 was obtained from Proteintech (cat. no. 24895–1-AP).

Techniques: Expressing, Western Blot, Control, Reverse Transcription Polymerase Chain Reaction

Journal: PLoS Pathogens

Article Title: H1N1 influenza virus infection results in adverse pregnancy outcomes by disrupting tissue-specific hormonal regulation

doi: 10.1371/journal.ppat.1006757

Figure Lengend Snippet: Hormone expression in sera was quantified via ELISA in uninfected and infected non-pregnant mice for A. progesterone; C. prostaglandin F2α (PGF2α); and D. prostaglandin E2 (PGE2). Cytokine and chemokine expression in sera was quantified via Bio-Rad 23-plex assay in B. uninfected pregnant (E16) (N = 5) and non-pregnant mice (N = 5); E. uninfected and infected pregnant (E16; 4 d.p.i) (N = 5 per group); and F. infected (4 d.p.i) non-pregnant and pregnant (E16) mice (N = 5 per group). Student’s t-test was performed between selected groups and significance noted above asterisk brackets. Inf.: infected; Uninf: uninfected; NP: non-pregnant; P: pregnant mice.

Article Snippet: Progesterone, prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2) were quantified via ELISA kits from ALPCO (Salem, NH).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Infection, Plex Assay

Journal: PLoS Pathogens

Article Title: H1N1 influenza virus infection results in adverse pregnancy outcomes by disrupting tissue-specific hormonal regulation

doi: 10.1371/journal.ppat.1006757

Figure Lengend Snippet: Hormone expression in lung lysates was quantified via ELISA in uninfected (N = 6) and infected (N = 5) non-pregnant and pregnant mice for A. progesterone; B. prostaglandin F2α (PGF2α); and C. prostaglandin E2 (PGE2). Cytokine and chemokine expression in sera was quantified in lung lysates of D. uninfected pregnant (E16) (N = 8) and non-pregnant mice (N = 6); E. infected pregnant (4 d.p.i., E16) (N = 5) and non-pregnant mice (N = 5). Student’s t-test was performed between selected groups and significance noted above asterisk brackets. Abbreviations for groups are as described in .

Article Snippet: Progesterone, prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2) were quantified via ELISA kits from ALPCO (Salem, NH).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Infection

Journal: PLoS Pathogens

Article Title: H1N1 influenza virus infection results in adverse pregnancy outcomes by disrupting tissue-specific hormonal regulation

doi: 10.1371/journal.ppat.1006757

Figure Lengend Snippet: Placental lysates were isolated from uninfected and infected pregnant mice (E16). Hormone expression was quantified via ELISA for A. progesterone (N = 6 per group); B. prostaglandin F2α (PGF2α) (N = 9, uninfected, N = 7, infected) and C. prostaglandin E2 (PGE2) (N = 9 per group). Student’s t-test was performed between selected groups and significance noted above asterisk brackets.

Article Snippet: Progesterone, prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2) were quantified via ELISA kits from ALPCO (Salem, NH).

Techniques: Isolation, Infection, Expressing, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Impact of Mechanical Load on the Expression Profile of Synovial Fibroblasts from Patients with and without Osteoarthritis

doi: 10.3390/ijms20030585

Figure Lengend Snippet: Impact of static compressive force application on the expression of proinflammatory genes. Gene expression and protein secretion of ( a ) TNF-α, ( b ) COX-2/PG-E2 and ( c ) IL-6 of N-SF and OA-SF after 48 h with or without static compressive force application. AU: arbitrary units; RT-qPCR: n = 9; ELISA: n = 6. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤0.001. Statistics: Welch-corrected ANOVA with Games-Howell post-hoc-tests.

Article Snippet: For quantification of tumour necrosis factor α (TNFα) and prostaglandin E2 (PGE2), interleukin-6 (IL-6), collagen-1 (COL-1) and fibronectin (FN-1) protein secretion into the synovial fibroblast supernatant, we used commercially available ELISA kits according to the manufacturers’ instructions (TNFα: EK0525, Boster Biological Technology; PGE2: 514010; Cayman chemical; IL-6: EK0410 Boster Biological Technology; COL-1: ab210966, Abcam; FN-1: EK0349, Boster Biological Technology).

Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay